Alignment of P1A ATPases (K+)

The alignment was performed on The Danish Biotechnological Database with the programme CLUSTALW with default settings. The alignment of the N- and C-terminal regions was refined by hand.
The grouping of P-type ATPases and the nomenclature is adopted from the work described in Axelsen, K.B. & Palmgren, M.G. (1998) J. Mol. Evol. 46: 84-101.
The sequence numbers refer to the position of the sequence in a phylogenetic tree of P-type ATPases.
The sequences with sequence numbers in parantheses are not included in the phylogenetic tree, but show more than 90 % sequence identity with the sequence with the specified number.
The number and positions of transmembrane helices are predicted with the TMHMM server at The Center for Biological Sequence Analysis and the method of von Heijne, and might, therefore, be different in the proteins in vivo.
The segments a-h conserved in all P-type ATPases are the amino acid stretches used for the phylogenetic analysis.
Amino acids in the alignment shown in CAPITALS are present in 83% (5 of 6) of the P1A ATPases. 
Number Accession    Organism                 Name
used   number                                            1                                                                                                    100 101                                                                                                  200 201                                                                                                  300 301                                                                                                  400 401                                                                                                  500 501                                                                                                  600 601                                                                                                  700 701             719 
  20   P03960  Escherichia coli            KdpB/f683     .................... msrkqlalfeptlvvqAlke avkKLnPqaqwrNPVMFiVw iGsllTtcisiamasga..m pgnalfsaaisgwLwiTVLF ANFAEalAEGRsKAQAnsLk gvkktafArklrepkygaaa dkvpadqLrkGDiVlVeaGd iIPcDGEvieGgASVDESAI TGESAPVirEsGgDfas.VT GGTrilSDwlviecsvnpGe tFlDRMIamVEGAqRrKTPN EIALtiLLiaLTivFLlata TLwPfsawggnavsvtvLvA LLVCLIPTTIGgLLSAIGvA GMsRmlgaNViAtSGrAVEa aGDVdvLlLDKTGTITlGNR qAseFiPaqGvdektladaA qlaSlaDeTPEGrSIViLAk qrfnlrerd.vqslhatfvp FTAqsRMSGinidn....rm irKGsvdairrhVeanGGhf PtdvdqkvdqVarqGaTPL. ....vVvegsrvlGViaLKD ivKgGikERFaqLRkMGikT VMiTGDNrlTAaaIAaEAGV DdFlAEAtPEaKlalIrqyQ aeGrLVAMTGDGTNDAPALA QAdVavAMNSGTqAAKEAgN MVDLDSnPTKLiEVVhIGKQ mLmTRGsLTTFSIANDvAKY FAIiPAaFaatyPqlnaLNi McLhSPdSAiLSAvIFNALI IvfLIPlAlkGVsykpltas AmLrRNlwiYGlGGllVPFi gIKvIDLlltvcglv....
  21   P96370  Mycobacterium tuberculosis  KdpB/Rv1030   mmiarmetsataaaatsapr lrlakrslfdpmivrsAlpq slrKLaPrvqarNPVMlvVl vGaviTtlaflrdlass..t aqenvfnglvaafLwfTVLF ANFAEamAEGRGKAQAaaLr kvrsetmAnrrta...agni esvpssrLdldDvVeVsaGe tIPsDGEiieGiASVDESAI TGESAPVirEsGgDrsa.VT GGTvvlSDrivvriTakqGq tFiDRMIaLVEGAaRqqTPN EIALniLLagLTiiFLlavv TLqPfaiysgggqrvvvLvA LLVCLIPTTIGaLLSAIGIA GMDRlvqhNVlAtSGrAVEa aGDVntLlLDKTGTITlGNR qAteFvPinGvsaeavadaA qlsSlaDeTPEGrSIVvLAk defglrardegvmsharfvp FTAeTRMSGvdlaevsgirr irKGAaaavmkwVrdhGGhp teevgaivdgissgGgTPLv vaewtdnssaraiGVvhLKD ivKvGirERFdemRrMsirT VMiTGDNpaTAkaIAqEAGV DdFlAEAtPEDKlalIkrEQ qgGrLVAMTGDGTNDAPALA QAdVGvAMNtGTqAArEAgN MVDLDSdPTKLiEVVeIGKQ LLiTRGaLTTFSIANDvAKY FAIiPAmFvglyPvldkLNv MaLhSPrSAiLSAvIFNALv IvaLIPlAlrGVrfraesas AmLrRNlliYGlGGlvVPFi gIKlvDLvIvalgvs....
   -   Q9RZP0  Deinococcus radiodurans     KdpB/DRB0083  .................mtt apqpkqsifspelvrgAvra sfaKLsPrdqarNPVMFvVy lGtalTayltvanlvtg..k pwg..yelaitllLllTVLF ANFAEgmAEaRGKAQAasLr saredvkArrlvn....gqe elvagtaLerGDlVvVeaGe mIPaDGEiveGlASVDESAI TGESAPVirEaGtDhsg.VT GGTkvlSDriviqiTsgaGe sFlDRMIaLVEGAsRqKTPN EIALsiLLsgLTliFLlavv TLyPftvyagapasavtLiA LLVCLIPTTIGgLLpAIGIA GMDRalqaNViAkSGkAVEv aGDVdvLlLDKTGTITiGNR mAtkFhPlpGvteaelakaA llsSlaDpTPEGkSIVaLAr qlgvdapep....agaefie FTAqTRMSGvdfpg....ts irKGAgsrisalaqerGGql PpelaaitdeVsrqGaTPL. ....tViendrllGVvaLsD iiKpGirERFeqLRrMGlrT VMiTGDNplTAeaIAkEAGV DgFlAEAtPEDKmemIkqEQ asGkLVAMmGDGTNDAPALA QAdVGlAMNSGTqAAKEAgN MVDLDSdPTKLlEVVeIGKg LLiTRGaLTTFSIANDvAKY FAIlPAlFvtayPqlgvLNv MgLhSPtSAvLSAvIFNALI IpvLIPlAlrGVpyqpmsag AlLnRNllvYGgGGilVPFi aIKlIDLlIgglms.....
  22   P73867  Synechocystis PCC6803       KdpB/slr1729  .....mnststvrqpggprq qrrhtpkanlsdlyqrAfke awvKLnPkimvkNPVMFmVw vGtliTgmltlnpnlfg.vt gtsamfnglvtviLlfTVLF ANFAEavAEGRGKAQAdaLr stqtqtyAqrila...dgsl emvssthLrkGDrivVsvGd iIPaDGEvleGvASVDESAI TGESAPVlkEpGsDvassVT GGTriiSDeliirvTadpGk gFiDRMIdLVEGAeRsKTPN EIALtvLLavLTlvFLivva TLpPpanyidspvsitlLiA LLVaLIPTTIGgLLSAIGIA GMDRvaqfNVvAtSGrAVEa cGDintLvLDKTGTITlGNR lAetFlPvnGhslkevaaiA laaSifDtTPEGkSIVrLAe kmgatldfd..rqq.aegve FsArTRMSGtdlpdg...se vrKGAvdairgfVrsrGGks PtgldeaygkVshlGgTPL. ....aVcrndeiyGViyLKD iiKpGiqERFnqLRrMGvrT VMlTGDNriTAsvIAkEAGV DdFiAEAtPEDKiqvIqqEQ aaGkLVAMTGDGTNDAPALA QAnVGlAMNSGTqAAKEAaN MVDLDSdPTKLidlVtIGKQ LLiTRGaLTTFSlANDiAKY FAIiPAmFagig..igaLNi MdLkSPqSAvLSAlIyNALI IpaLIPlAlrGVkfrplsad qlLqRNilvYGlGGiiVPFv aIKlIDLgIsll.......
   -   O32328  Clostridium acetobutylicum  KdpB          .................... mkskkskfitkdilkeAiie sfkKikPkymmkNPVMFvVe vGffvTilltifpsifgdkg hnlrvynlivtiiLfiTVLF ANFAEsvAEGRGKAQAdaLk ktrkdtiAkligk...dgsi ktinaneLkkGDvVlVenGd vIPnDGEvvdGvASVDESAI TGESAPVmkEpGgDfas.VT GGTkvvSDwlkveiTatpGe sFlDkMInLVEGAsRqKTPN EIALntiLvsLTliFLivlv aLyPmatytgvkipmstLir LLVCLIPTTIGaLLSAIGIA GMDRvtrfNViAmSGkAVEa cGDVdtmiLDKTGTITyGNR lAadFitvgGadkqklidys vmcSlkDdTPEGkSIVeLgk qlgitidtk..kyesiefee FTAqTRMSGikleng...ta vkKGAydaikkrVqelkGvi PkdldeavnkVaklGgTPL. ....vVcvdnkiyGViyLKD tvKpGlvERFerLReiGikT iMcTGDNplTAatIAkEAGV DgFiAEckPEDKieaIkkEQ deGkLVAMTGDGTNDAPALA QAdVGlAMNSGTtAAKEAaN MVDLDSdPTKvlEVVeIGKQ LLiTRGaLTTFSIANDvAKY FAIiPAiFtiaiPkmqlmNi MhLstPySAiLSAlIFNAiI IpaLIPiAmkGVkyrpmkse AlLlRNmivfGfGGiiVPFv gIKiIDmiItpmvrilnlg
   -   Q9XBA9  Staphylococcus aureus       KdpB          .................... .maettkifeshlvkqAlkd svlKLyPvymikNPiMFvVe vGmllalgltiypdlfhqes vs.rlyvfsifiiLllTlvF ANFsEalAEGRGKAQAnaLr qtqtemkArrikq...dgsy emidasdLkkGhiVrVatGe qIPnDGkvikGlAtVDESAI TGESAPVikEsGgDfd.nVi GGTsvaSDwleveiTsepGh sFlDkMIgLVEGAtRkKTPN EIALftLLmtLTiiFLvvil TmyPlakflnfnlsiamLiA LaVCLIPTTIGgLLSAIGIA GMDRvtqfNilAkSGrsVEt cGDVnvLiLDKTGTITyGNR mAdaFiPvksssferlvkaA yesSiaDdTPEGrSIVkLAy kqhidlp.....qevgeyip FTAeTRMSGvkftt....re vyKGApnsmvkrVkeaGGhi PvdldalvkgVskkGgTPLv .....VledneilGViyLKD viKdGlvERFreLReMGieT VMcTGDNelTAatIAkEAGV DrFvAEckPEDKinvIreEQ akGhiVAMTGDGTNDAPALA eAnVGlAMNSGTmsAKEAaN liDLDSnPTKLmEVVlIGKQ LLmTRGsLTTFSIANDiAKY FAIlPAmFmaamPamnhLNi MhLhSPeSAvLSAlIFNALI IvlLIPiAmkGVkfkgastq tiLmkNmlvYGlGGmiVPFi gIKlIDLiIqlfv......
Identical in 5 of the 6 sequences                        -------------------- ----------------A--- ---KL-P-----NPVMF-V- -G---T-------------- -------------L--TVLF ANFAE--AEGRGKAQA--L- -------A------------ -------L--GD-V-V--G- -IP-DGE---G-ASVDESAI TGESAPV--E-G-D----VT GGT---SD------T---G- -F-D-MI-LVEGA-R-KTPN EIAL--LL--LT--FL---- TL-P-------------L-A LLVCLIPTTIG-LLSAIGIA GMDR----NV-A-SG-AVE- -GDV--L-LDKTGTIT-GNR -A--F-P--G---------A ---S--D-TPEG-SIV-LA- -------------------- FTA-TRMSG----------- --KGA-------V---GG-- P---------V---G-TPL- -----V-------GV--LKD --K-G--ERF--LR-MG--T VM-TGDN--TA--IA-EAGV D-F-AE--PEDK---I--EQ --G-LVAMTGDGTNDAPALA QA-VG-AMNSGT-AAKEA-N MVDLDS-PTKL-EVV-IGKQ LL-TRG-LTTFSIAND-AKY FAI-PA-F----P----LN- M-L-SP-SA-LSA-IFNALI I--LIP-A--GV-------- --L-RN---YG-GG--VPF- -IK-IDL-I----------
Identical in all 6 sequences                             -------------------- ----------------A--- ---K--P-----NP-M--V- -G------------------ -------------L--T--F ANF-E--AE-R-KAQA--L- -------A------------ -------L-------V--G- -IP-DG----G-A-VDESAI TGESAPV--E-G-D----V- GGT---SD----------G- -F-D-MI--VEGA-R--TPN EIAL---L--LT--FL---- ---P-------------L-- L-V-LIPTTIG-LL-AIG-A GM-R----N--A-SG--VE- -GD-----LDKTGTIT-GNR -A--F--------------- ---S--D-TPEG-SIV-L-- -------------------- F-A--RMSG----------- --KG-------------G-- --------------G-TPL- -------------GV--L-D --K-G--ERF---R-----T -M-TGDN--TA--IA-EAGV D-F-AE--PE-K---I---Q --G--VAM-GDGTNDAPALA -A-V--AMN-GT--A-EA-N --DLDS-PTK----V-IGK- -L-TRG-LTTFS-AND-AKY FAI-PA-F----------N- M-L--P-SA-LSA-I-NA-- I--LIP-A--GV-------- --L--N----G-GG--VPF- -IK--D-------------
Conserved segments in all P-type ATPases                                                                                                                                                                       aaaaaaaaaaaaaaaaa aaaaaaaa      bbbbbb bbbbbbbbbbbbbb     c cccccccccccccccccccc ccccc                                                   dddddd dddddddddddddddddd           eeeeeeeeeeee eeeeeeeeeeeeeeeeeeee eeee                                                                                                                              ffffffffffffffff ffffffffffffffffffff ffffffffffffffffffff f gggggggggggggggggg gggggggggggggggggggg gggggggg  hhhhhhhhhh hhhhhhhhhhhhhhhhhhhh h                                                                                                                           
Transmembrane helices                                                                                           1111111 11111111111111           2222222222222222 222                                                                                                                                                   33333333333333333 333333        444444 444444444444                                                                                                                                                                                                                                                                                                                                         555555555555555 55555                         66666666666 66666666                   77777777777777 777777777
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