Alignment of P3B ATPases(Mg2+)
The alignment was performed on The Danish Biotechnological Database with the programme CLUSTALW with default settings. The alignment was refined by hand.
The grouping of P-type ATPases and the nomenclature is adopted from the work described in
Axelsen, K.B. & Palmgren, M.G. (1998) J. Mol. Evol. 46: 84-101.
The sequence numbers refer to the position of the sequence in a phylogenetic tree of P-type ATPases.
The sequences with sequence numbers in parantheses are not included in the phylogenetic tree, but show more than 90 % sequence identity with the sequence with the specified number.
The positions of the transmembrane helices are predicted with the method of von Heijne. They might, therefore, be slightly different in the proteins in vivo.
The segments a-h conserved in all P-type ATPases are the amino acid stretches used for the phylogenetic analysis.
Amino acids in the alignment shown in CAPITALS are present in all (3 of 3) the P3B ATPases.
Number Accession Organism Name
used number 1 100 101 150 200 201 250 300 301 350 400 401 450 500 501 550 600 601 650 700 701 750 800 801 850 900 901 927
58 P22036 Salmonella typhimurium MgtB ...........mtdmnienr klnR.PasendkqhkkvfPi eae......AfhspEETLAR LNSHRQGLTIEEASERLKVY GRNEVAHEQVPPALIQLLQA FNNPFIYVLMALAGVSFITD YWLPLRRGEETDLTGVLIIL TMVSLSGLLRFWQEFRTNRA AQALKKMVRTTATVLRrgpg nigavqeEIPIEELVPGDVV FLAAGDLVPADVRLLASRDL FISQSILSGESLPVEKydvm advagkdseqlpdkdkslLD LGNICLMGTNVTSGRAQAVV VATGSRTWFGSLAKSIVG.T RTQTAFDRGVNSVSWLLIRF MLIMvpvvllingfskgdwv easLFALAVAVGLTPEMLPM IVSSNLAKGAIAMSRRKVIV KRLNAIQNFGAMDVLCTDKT GTLTQDNIFLEHHLDVSGVK SSRVLMLAWLNSSSQSGARN VMDRAILRFGEGRIAPSTKA RFIKRDELPFDFVRRRVSVL VEDAQHGDRCLICKGAVEEM MMVathlRegdrvvaLtetr relllakTedyNaQGfRVll iATrkLdgsgnnptlsveDE TELTIEGMLTFLDPPKESAG KAIAALRDNGVAVKVLTGDN PVVTARICLEVGIDTHDILT GTQVEAMSDAELASEVEKRA VFARLTPLQKTRILQALQKN GHTVGFLGDGINDAPALRDA DVGISVDSAADIAKESSDII LLEKDLMVLEEGVIKGRETF GNIIKYLNMTASSNFVNVFS VLVASAFIPFLPMLAIHLLI QNLMYDISQLSLPWDKMDKE FLRKPRKWDAKNIGRFMLWI GPTSSIFDITTFALMWYVFA ANNVEAQALFQSGWFIEGLL SQTLVVHMLRTQKIPFIQSR ATLPVLLTTGLIMAIGIYIP FSPLGAMVGLEPLPLSYFPW LVATLLSYCLVAQGMKRFYI KRFGQWF
59 P39168 Escherichia coli MgtA /o898 ....mfkeiftrlirhlpsr lvhRdPlpgaqqtvntvvPp slsahclkmAvmpeEELWKT FDTHPEGLNQAEVESAREQH GENKLPAQQPSPWWVHLWVC YRNPFNILLTILGAISYATE ...........DLFAAGVIA LMVAISTLLNFIQEARSTKA ADALKAMVSNTATVLRvind kgengwlEIPIDQLVPGDII KLAAGDMIPADLRILQARDL FVAQASLTGESLPVEKaatt rqpeh..........snpLE CDTLCFMGTTVVSGTAQAMV IATGANTWFGQLAGRVSEQE SEPNAFQQGISRVSMLLIRF MLVMaaggavnqwlhqrrlv gsaLFALSVAVGLTPEMLPM IVTSTLARGAVKLSKQKVIV KHLDAIQNFGAMDILCTDKT GTLTQDKIVLENHTDISGKT SERVLHSAWLNSHYQTGLKN LLDTAVLEGTDEESARSLAS RWQKIDEIPFDFERRRMSVV VAENTEHHQ.LVCKGALQEI LNVcsqvRhngeivpLddim lrkikrvTdtlNrQGlRVva vATkyLparegd..yqraDE SDLILEGYIAFLDPPKETTA PALKALKASGITVKILTGDS ELVAAKVCHEVGLDAGEVVI GSDIETLSDDELANLAQRTT LFARLTPMHKERIVTLLKRE GHVVGFMGDGINDAPALRAA DIGISVDGAVDIAREAADII LLEKSLMVLEEGVIEGRRTF ANMLKYIKMTASSNFGNVFS VLVASAFLPFLPMLPLHLLI QNLLYDVSQVAIPFDNVDDE QIQKPQRWNPADLGRFMIFF GPISSIFDILTFCLMWWVFH ANTPETQTLFQSGWFVVGLL SQTLIVHMIRTRRVPFIQSC ASWPLMIMTVIVMIVGIALP FSPLASYLQLQALPLSYFPW LVAILAGYMTLTQLVKGFYS RRYGWQ.
60 P36640 Salmonella typhimurium MgtA mlkiitrqlfarlnrhlpyr lvhRdPlpgaqtavnatiPp slserclkvAameqETLWRV FDTHPEGLNAAEVTRAREKH GENRLPAQKPSPWWVHLWVC YRNPFNILLTILGGISYATE ...........DLFAAGVIA LMVGISTLLNFVQEARSTKA ADALKAMVSNTATVLRvine ngenawlELPIDQLVPGDII KLAAGDMIPADLRIIQARDL FVAQASLTGESLPVEKvaat reprq..........nnpLE CDTLCFMGTNVVSGTAQAVV MATGAGTWFGQLAGRVSEQD NEQNAFQKGISRVSMLLIRF MLVMapvvliingytkgdww eaaLFALSVAVGLTPEMLPM IVTSTLARGAVKLSKQKVIV KHLDAIQNFGAMDILCTDKT GTLTQDKIVLENHTDISGKP SEHVLHCAWLNSHYQTGLKN LLDTAVLEGVDETAARQLSG RWQKIDEIPFDFERRRMSVV VAEDSNVHQ.LVCKGALQEI LNVctqvRhngdivpLddnm lrrvkrvTdtlNrQGlRVva vATkyLparegd..yqriDE SDLILEGYIAFLDPPKETTA PALKALKASGITVKILTGDS ELVAAKVCHEVGLDAGDVII GSDIEGLSDDALAALAARTT LFARLTPMHKERIVTLLKRE GHVVGFMGDGINDAPALRAA DIGISVDGAVDIAREAADII LLEKSLMVLEEGVIEGRRTF SNMLKYIKMTASSNFGNVFS VLVASAFLPFLPMLPLHLLI QNLLYDVSQVAIPFDNVDEE QIQKPQRWNPADLGRFMVFF GPISSIFDILTFCLMWWVFH ANTPETQTLFQSGWFVVGLL SQTLIVHMIRTRRLPFIQSR AAWPLMAMTLLVMVVGVSLP FSPLASYLQLQALPLSYFPW LIAILVGYMTLTQLVKGFYS RRYGWQ.
Identical in all 3 sequences -------------------- ---R-P------------P- ---------A----E----- ---H--GL---E-------- G-N--------P----L--- --NPF---L--L---S--T- -----------DL-----I- -MV--S-LL-F-QE-R---A AQALK-MV--TATVLR---- -------EIPI--LVPGD-- -LAAGD--PAD-R-L--RDL F--Q--L-GESLPVEK---- ------------------L- ----C-MGT-V-SG-AQA-V -ATG--TWFG-LA------- ----AF--G---VS-LLIRF ML-M---------------- ---LFAL-VAVGLTPEMLPM IV-S-LA-GA---S--KVIV K-L-AIQNFGAMD-LCTDKT GTLTQD-I-LE-H-D-SG-- S--VL--AWLNS--Q-G--N --D-A-L-------A----- R----DELPFDF-RRR-SV- V---------L-CKGA--E- -MV----R-------L---- -------T---N-QG-RV-- -AT--L------------DE --LTIEG---FLDPPKE--- -A--AL---G--VK-LTGD- --V-A--C-EVG-D------ G---E--SD--LA------- -FARLTP--K-RI---L--- GH-VGF-GDGINDAPALR-A D-GISVD-A-DIA-E--DII LLEK-LMVLEEGVI-GR-TF -N--KY--MTASSNF-NVFS VLVASAF-PFLPML--HLLI QNL-YD-SQ-S-P-D--D-E ---KP--W-----GRFM--- GP-SSIFDI-TF-LMW-VF- AN--E-Q-LFQSGWF--GLL SQTL-VHM-RT---PFIQS- A--P----T---M--G---P FSPL-----L--LPLSYFPW L-A-L--Y----Q--K-FY- -R-G---
Conserved segments in all P-type ATPases aaaaaaaaaaa aaaaaaaaaaaaaa bbbbbbbbbbbbbbbbbbb b ccccccccccccccc ccccccccccc dddddddddddddddddd dddddd eeee eeeeeeeeeeeeeeeeeeee eeeeeeeeeeee fff ffffffffffffffffffff ffffffffffffffffffff ffffffffffffff gggggggggggggggggggg gggggggggggggggggggg gggggg hhhhhhhhhhhh hhhhhhhhhhhhhhhhhhh
Transmembrane helices 11 1111111111111111111 222222222 222222222222 33333333 3333333333333 444444444444444444 55555555555555555 555 66666666 666666666666 777777 777777777777777777 8888888888888 888888 9 9999999999999999999 000000 00000000000000
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