Alignment of P3B ATPases(Mg2+)

The alignment was performed on The Danish Biotechnological Database with the programme CLUSTALW with default settings. The alignment was refined by hand.
The grouping of P-type ATPases and the nomenclature is adopted from the work described in Axelsen, K.B. & Palmgren, M.G. (1998) J. Mol. Evol. 46: 84-101.
The sequence numbers refer to the position of the sequence in a phylogenetic tree of P-type ATPases.
The sequences with sequence numbers in parantheses are not included in the phylogenetic tree, but show more than 90 % sequence identity with the sequence with the specified number.
The positions of the transmembrane helices are predicted with the method of von Heijne. They might, therefore, be slightly different in the proteins in vivo.
The segments a-h conserved in all P-type ATPases are the amino acid stretches used for the phylogenetic analysis.
Amino acids in the alignment shown in CAPITALS are present in all (3 of 3) the P3B ATPases.
Number Accession    Organism             Name
used   number                                        1                                                                                                    100 101                                              150                                                 200 201                                              250                                                 300 301                                              350                                                 400 401                                              450                                                 500 501                                              550                                                 600 601                                              650                                                 700 701                                              750                                                 800 801                                              850                                                 900 901                      927
  58   P22036    Salmonella typhimurium  MgtB        ...........mtdmnienr klnR.PasendkqhkkvfPi eae......AfhspEETLAR LNSHRQGLTIEEASERLKVY GRNEVAHEQVPPALIQLLQA FNNPFIYVLMALAGVSFITD YWLPLRRGEETDLTGVLIIL TMVSLSGLLRFWQEFRTNRA AQALKKMVRTTATVLRrgpg nigavqeEIPIEELVPGDVV FLAAGDLVPADVRLLASRDL FISQSILSGESLPVEKydvm advagkdseqlpdkdkslLD LGNICLMGTNVTSGRAQAVV VATGSRTWFGSLAKSIVG.T RTQTAFDRGVNSVSWLLIRF MLIMvpvvllingfskgdwv easLFALAVAVGLTPEMLPM IVSSNLAKGAIAMSRRKVIV KRLNAIQNFGAMDVLCTDKT GTLTQDNIFLEHHLDVSGVK SSRVLMLAWLNSSSQSGARN VMDRAILRFGEGRIAPSTKA RFIKRDELPFDFVRRRVSVL VEDAQHGDRCLICKGAVEEM MMVathlRegdrvvaLtetr relllakTedyNaQGfRVll iATrkLdgsgnnptlsveDE TELTIEGMLTFLDPPKESAG KAIAALRDNGVAVKVLTGDN PVVTARICLEVGIDTHDILT GTQVEAMSDAELASEVEKRA VFARLTPLQKTRILQALQKN GHTVGFLGDGINDAPALRDA DVGISVDSAADIAKESSDII LLEKDLMVLEEGVIKGRETF GNIIKYLNMTASSNFVNVFS VLVASAFIPFLPMLAIHLLI QNLMYDISQLSLPWDKMDKE FLRKPRKWDAKNIGRFMLWI GPTSSIFDITTFALMWYVFA ANNVEAQALFQSGWFIEGLL SQTLVVHMLRTQKIPFIQSR ATLPVLLTTGLIMAIGIYIP FSPLGAMVGLEPLPLSYFPW LVATLLSYCLVAQGMKRFYI KRFGQWF
  59   P39168    Escherichia coli        MgtA /o898  ....mfkeiftrlirhlpsr lvhRdPlpgaqqtvntvvPp slsahclkmAvmpeEELWKT FDTHPEGLNQAEVESAREQH GENKLPAQQPSPWWVHLWVC YRNPFNILLTILGAISYATE ...........DLFAAGVIA LMVAISTLLNFIQEARSTKA ADALKAMVSNTATVLRvind kgengwlEIPIDQLVPGDII KLAAGDMIPADLRILQARDL FVAQASLTGESLPVEKaatt rqpeh..........snpLE CDTLCFMGTTVVSGTAQAMV IATGANTWFGQLAGRVSEQE SEPNAFQQGISRVSMLLIRF MLVMaaggavnqwlhqrrlv gsaLFALSVAVGLTPEMLPM IVTSTLARGAVKLSKQKVIV KHLDAIQNFGAMDILCTDKT GTLTQDKIVLENHTDISGKT SERVLHSAWLNSHYQTGLKN LLDTAVLEGTDEESARSLAS RWQKIDEIPFDFERRRMSVV VAENTEHHQ.LVCKGALQEI LNVcsqvRhngeivpLddim lrkikrvTdtlNrQGlRVva vATkyLparegd..yqraDE SDLILEGYIAFLDPPKETTA PALKALKASGITVKILTGDS ELVAAKVCHEVGLDAGEVVI GSDIETLSDDELANLAQRTT LFARLTPMHKERIVTLLKRE GHVVGFMGDGINDAPALRAA DIGISVDGAVDIAREAADII LLEKSLMVLEEGVIEGRRTF ANMLKYIKMTASSNFGNVFS VLVASAFLPFLPMLPLHLLI QNLLYDVSQVAIPFDNVDDE QIQKPQRWNPADLGRFMIFF GPISSIFDILTFCLMWWVFH ANTPETQTLFQSGWFVVGLL SQTLIVHMIRTRRVPFIQSC ASWPLMIMTVIVMIVGIALP FSPLASYLQLQALPLSYFPW LVAILAGYMTLTQLVKGFYS RRYGWQ.
  60   P36640    Salmonella typhimurium  MgtA        mlkiitrqlfarlnrhlpyr lvhRdPlpgaqtavnatiPp slserclkvAameqETLWRV FDTHPEGLNAAEVTRAREKH GENRLPAQKPSPWWVHLWVC YRNPFNILLTILGGISYATE ...........DLFAAGVIA LMVGISTLLNFVQEARSTKA ADALKAMVSNTATVLRvine ngenawlELPIDQLVPGDII KLAAGDMIPADLRIIQARDL FVAQASLTGESLPVEKvaat reprq..........nnpLE CDTLCFMGTNVVSGTAQAVV MATGAGTWFGQLAGRVSEQD NEQNAFQKGISRVSMLLIRF MLVMapvvliingytkgdww eaaLFALSVAVGLTPEMLPM IVTSTLARGAVKLSKQKVIV KHLDAIQNFGAMDILCTDKT GTLTQDKIVLENHTDISGKP SEHVLHCAWLNSHYQTGLKN LLDTAVLEGVDETAARQLSG RWQKIDEIPFDFERRRMSVV VAEDSNVHQ.LVCKGALQEI LNVctqvRhngdivpLddnm lrrvkrvTdtlNrQGlRVva vATkyLparegd..yqriDE SDLILEGYIAFLDPPKETTA PALKALKASGITVKILTGDS ELVAAKVCHEVGLDAGDVII GSDIEGLSDDALAALAARTT LFARLTPMHKERIVTLLKRE GHVVGFMGDGINDAPALRAA DIGISVDGAVDIAREAADII LLEKSLMVLEEGVIEGRRTF SNMLKYIKMTASSNFGNVFS VLVASAFLPFLPMLPLHLLI QNLLYDVSQVAIPFDNVDEE QIQKPQRWNPADLGRFMVFF GPISSIFDILTFCLMWWVFH ANTPETQTLFQSGWFVVGLL SQTLIVHMIRTRRLPFIQSR AAWPLMAMTLLVMVVGVSLP FSPLASYLQLQALPLSYFPW LIAILVGYMTLTQLVKGFYS RRYGWQ.
Identical in all 3 sequences                         -------------------- ---R-P------------P- ---------A----E----- ---H--GL---E-------- G-N--------P----L--- --NPF---L--L---S--T- -----------DL-----I- -MV--S-LL-F-QE-R---A AQALK-MV--TATVLR---- -------EIPI--LVPGD-- -LAAGD--PAD-R-L--RDL F--Q--L-GESLPVEK---- ------------------L- ----C-MGT-V-SG-AQA-V -ATG--TWFG-LA------- ----AF--G---VS-LLIRF ML-M---------------- ---LFAL-VAVGLTPEMLPM IV-S-LA-GA---S--KVIV K-L-AIQNFGAMD-LCTDKT GTLTQD-I-LE-H-D-SG-- S--VL--AWLNS--Q-G--N --D-A-L-------A----- R----DELPFDF-RRR-SV- V---------L-CKGA--E- -MV----R-------L---- -------T---N-QG-RV-- -AT--L------------DE --LTIEG---FLDPPKE--- -A--AL---G--VK-LTGD- --V-A--C-EVG-D------ G---E--SD--LA------- -FARLTP--K-RI---L--- GH-VGF-GDGINDAPALR-A D-GISVD-A-DIA-E--DII LLEK-LMVLEEGVI-GR-TF -N--KY--MTASSNF-NVFS VLVASAF-PFLPML--HLLI QNL-YD-SQ-S-P-D--D-E ---KP--W-----GRFM--- GP-SSIFDI-TF-LMW-VF- AN--E-Q-LFQSGWF--GLL SQTL-VHM-RT---PFIQS- A--P----T---M--G---P FSPL-----L--LPLSYFPW L-A-L--Y----Q--K-FY- -R-G---
Conserved segments in all P-type ATPases                                                                                                                                                                                                                   aaaaaaaaaaa aaaaaaaaaaaaaa        bbbbbbbbbbbbbbbbbbb b                         ccccccccccccccc ccccccccccc                                                      dddddddddddddddddd dddddd          eeee eeeeeeeeeeeeeeeeeeee eeeeeeeeeeee                                                                                                                                                        fff ffffffffffffffffffff ffffffffffffffffffff ffffffffffffff                            gggggggggggggggggggg gggggggggggggggggggg gggggg  hhhhhhhhhhhh hhhhhhhhhhhhhhhhhhh                                                                                                                                                                                                                           
Transmembrane helices                                                                                                                                      11 1111111111111111111             222222222 222222222222                                                                                                                                                                        33333333 3333333333333          444444444444444444                                                                                                                                                                                                                                                                                                                                                                                              55555555555555555 555         66666666 666666666666                       777777 777777777777777777          8888888888888 888888             9 9999999999999999999                000000 00000000000000              


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